Nuclear DNA content and chromosome number determination in a Sahel medicinal plant, Combretum micranthum G. Don

Download
(PDF; 2.0 MB)
Cite
Feedback

Abstract

Combretum micranthum G. Don, also known as kinkéliba, is native to West Africa where the plant grows wild as a shrub, liana, or tree and is valued for its numerous attributes including health and nutritional benefits. Despite this, genetic information commensurate with its economic value is lacking. The aim of this study was to determine the nuclear DNA content and chromosome numbers of C. micranthum germplasm from eastern Senegal, West Africa. Genome size estimates were determined, for the first time, and chromosome number was analyzed using actively growing root tips. The mean, median, minimum, and maximum genome size estimates were 1587.44 Mb, 1540.85 Mb, 1472.24 Mb, and 2146.66 Mb, respectively. Cytological analysis revealed a chromosome number of 2 n = 2x = 26. The results of this investigation can be useful for structural and functional genomics, genome sequencing endeavors, and phylogenic studies as well as breeding and domestication programs.

  • Citation: Sakhanokho, Hamidou F.; Islam-Faridi, Nurul; Smith, Barbara J. 2022. Nuclear DNA content and chromosome number determination in a Sahel medicinal plant, Combretum micranthum G. Don . Journal of Crop Improvement. 36(6): 866-874. https://doi.org/10.1080/15427528.2022.2030447.
  • Keywords: Cytogenetics, Combretum spp, flow cytometry, genome size, kinkeliba, quinqueliba
  • Posted Date: December 2, 2022
  • Modified Date: December 5, 2022

    • Print Publications Are No Longer Available

    In an ongoing effort to be fiscally responsible, the Southern Research Station (SRS) will no longer produce and distribute hard copies of our publications. Many SRS publications are available at cost via the Government Printing Office (GPO). Electronic versions of publications may be downloaded, printed, and distributed.

    Publication Notes

    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
    • Our online publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact the SRS webmaster if you notice any errors which make this publication unusable.
    • To view this article, download the latest version of Adobe Acrobat Reader.