White oak (Quercus alba L.) microsatellite markers for genetic diversity studies
We screened and validated microsatellite DNA markers (i.e., simple sequence repeats, SSRs) from transcriptome sequences of white oak (Quercus alba L.). Of 84 PCR primer pairs previously identified and designed, we found 23 pairs that amplified white oak genomic DNA consistently and were polymorphic among three DNA samples. Subsequently, 16 of these 23 primer pairs were amplified across 225 white oak trees sampled from naturally regenerated stands on the Daniel Boone National Forest in southeastern Kentucky. Population estimates of heterozygosity and total and effective allele numbers averaged 66.4%, 11.3, and 5.5, respectively. These 16 SSR markers proved to be highly polymorphic and provided informative data on the genetic diversity of the white oaks within these stands.