Cellular response of loblolly pine to wound inoculation with bark beetle-associated fungi and chitosan

  • Authors: Klepzig, Kier D.; Walkinshaw, Charles H.
  • Publication Year: 2003
  • Publication Series: Research Paper (RP)
  • Source: Res. Pap. SRS-30.Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 9p.

Abstract

We inoculated loblolly pines with bark beetle-associated fungi and a fungal cell wall component, chitosan, known to induce responses in some pines and many other plants. Trees in Florida were inoculated with Leptographium procerum, L. terebrantis, Ophiostoma minus, or chitosan. Trees in Louisiana were inoculated with O. minus, Entomocorticium sp. A, or Ceratocystiopsis ranaculosus. In both Florida and Louisiana, mechanical wounds served as controls. Treatment responses were sampled after 3 weeks, and all produced uniform responses across trees. Inoculations with E. sp. A and C. ranaculosus appeared similar to controls. Inoculations with L. procerum produced slightly higher levels of host damage. Loblolly pine responded similarly to chitosan and pathogenic bark beetle-associated fungi (O. minus and L. terebrantis), producing high levels of phenolic compounds and cell hydrolysis in the callus. In addition, callus inoculated with O. minus appeared extremely disrupted and "stringy." Chitosan inoculations resulted in no hydrolysis, but produced extremely high levels of phenolics deposition, as well as noticeable periderm formation. Our results reveal possible morphological mechanisms for pine secondary response to these fungi and suggest that chitosan may have potential as a stable material for testing variability in this response.

  • Citation: Klepzig, Kier D.; Walkinshaw, Charles H. 2003. Cellular response of loblolly pine to wound inoculation with bark beetle-associated fungi and chitosan. Res. Pap. SRS-30.Asheville, NC: U.S. Department of Agriculture, Forest Service, Southern Research Station. 9p.
  • Keywords: Leptographium, Ophiostoma, resin, resistance, southern pine beetle
  • Posted Date: April 1, 1980
  • Modified Date: August 22, 2006
  • Print Publications Are No Longer Available

    In an ongoing effort to be fiscally responsible, the Southern Research Station (SRS) will no longer produce and distribute hard copies of our publications. Many SRS publications are available at cost via the Government Printing Office (GPO). Electronic versions of publications may be downloaded, printed, and distributed.

    Publication Notes

    • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
    • Our online publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact the SRS webmaster if you notice any errors which make this publication unusable.
    • To view this article, download the latest version of Adobe Acrobat Reader.