Abstract
The industrial production of palm oil concurrently generates a substantial amount of empty fruit bunch (EFB) fibers that could be used as a feedstock in a lignocellulose based biorefinery. Lignin byproducts generated by this process may offer opportunities for the isolation of value-added products, such as
p-hydroxybenzoate (
pBz), to help offset operating costs. Analysis of the EFB lignin by nuclear magnetic resonance (NMR) spectroscopy clearly revealed the presence of bound acetate and
pBz, with saponification revealing that 1.1 wt% of the EFB was
pBz; with a lignin content of 22.7 %, 4.8 % of the lignin is
pBz that can be obtained as a pure component for use as a chemical feedstock. Analysis of EFB lignin by NMR and derivatization followed by reductive cleavage (DFRC) showed that
pBz selectively acylates the γ-hydroxyl group of S units. This selectivity suggests that
pBz, analogously with acetate in kenaf,
p-coumarate in grasses, and ferulate in a transgenic poplar augmented with a feruloyl-CoAmonolignol transferase (FMT), is incorporated into the growing lignin chain via its γ-
p-hydroxybenzoylated monolignol conjugate. Involvement of such conjugates in palm lignification is proven by the observation of novel phydroxybenzoylated non-resinol β–β-coupled units in the lignins. Together, the data implicate the existence of phydroxybenzoyl- CoA:monolignol transferases that are involved in lignification in the various willows (
Salix spp.), poplars and aspen (
Populus spp., family Salicaceae), and palms (family Arecaceae) that have
p-hydroxybenzoylated lignins. Even without enhancing the levels by breeding or genetic engineering, current palm oil EFB ‘wastes’ should be able to generate a sizeable stream of
p-hydroxybenzoic acid that offers opportunities for the development of value-added products derived from the oil palm industry.
Keywords
Lignin acylation,
Transferase,
NMR,
DFRC method,
Poplar,
p-Hydroxybenzoic acid,
Monolignol
Citation
Lu, Fachuang; Karlen, Steven D.; Regner, Matt; Kim, Hoon; Ralph, Sally A.; Sun, Run-Cang; Kuroda, Ken-ichi; Augustin, Mary Ann; Mawson, Raymond; Sabarez, Henry; Singh, Tanoj; Jimenez-Monteon, Gerardo; Zakaria, Sarani; Hill, Stefan; Harris, Philip J.; Boerjan, Wout; Wilkerson, Curtis G.; Mansfield, Shawn D.; Ralph, John. 2015. Naturally
p-Hydroxybenzoylated Lignins in Palms. BioEnergy Research. 8(3): 934-952.