Global and comparative protein profiles of the pronotum of the southern pine beetle, Dendroctonus frontalis

  • Author(s): Pechanova, O.; Stone, W.D.; Monroe, W.; Nebeker, T.E.; Klepzig, K.D.; Yuceer, C.
  • Date: 2008
  • Source: Insect Molecular Biology, Vol. 17(3): 261-277
  • Station ID: --


The southern pine beetle ( Dendroctonus frontalis Zimmermann) kills all pines within its range and is among the most important forest pest species in the US. Using a specialized mycangium surrounded by gland cells in the pronotum, adult females culture, transport, and inoculate two fungi into beetle galleries during oviposition. These fungal symbionts, to varying degrees, exclude antagonistic fungi and provide nutrients to larvae. However, the mechanisms (e.g. secreted antibiotic chemicals or nutrients, proteins or pathways) by which this relationship is maintained are not known. Here we present the first global and differential proteome profile of the southern pine beetle pronotum. Two-dimensional polyacrylamide electrophoresis, tandem mass spectrometry, and database searches revealed that the majority of pronotal proteins were related to energy-yielding metabolism, contractile apparati, cell structure, and defence. The identified proteins provide important insights into the molecular and biochemical processes of, and candidates for functional genomics to understand mycangia and pronotum functions in, the southern pine beetle.

  • Citation: . . Global and comparative protein profiles of the pronotum of the southern pine beetle, Dendroctonus frontalis. Insect Molecular Biology, Vol. 17(3): 261-277.

Requesting Publications

You can order print copies of our publications through our publication ordering system. Make a note of the publication you wish to request, and visit our Publication Order Site.

Publication Notes

  • This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
  • Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact the SRS webmaster if you notice any errors which make this publication unuseable.
  • To view this article, download the latest version of Adobe Acrobat Reader.