Forest Products Laboratory

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Title: 2-deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis

Author: Sreenath, Hassan K.; Jeffries, Thomas W.

Date: 1998

Source: Twentieth symposium on biotechnology for fuels and chemicals : proceedings of the twentieth symposium on biotechnology for fuels and chemicals held May 3-7, 1998, in Gatlinburg, Tennessee. Totowa, N.J. : Humana Press, 1999. Applied biochemistry and biotechnology, 0273-2280 ; vols. 77-79.:p. 211-222.

Description: The glucose analog 2-deoxyglucose (2-DOG) has been used to obtain mutants derepressed for pentose metabolism. Some researchers have used 2-DOG alone whereas others have used it in the presence of a glucoserepressible carbon source. We examined both methods and screened mutant strains for improved use of xylose in the presence of glucose. Pichia stipitis mutants selected for growth on D-xylose in the presence of 2-DOG used xylose from a 1:1 glucose: xylose mixture more rapidly than did their parents. One of these mutants, FPL-DX26, completely consumed xylose in the presence of glucose and produced 33 g/L ethanol in 45 h from 80 g/L of this sugar mixture. Mutants selected for growth on 2-DOG alone did not show significant improvement. Selection for growth on D-xylose in the presence of 2-DOG has been useful in developing parental strains for further genetic manipulation.

Key Words: Fermentation, Pichia stipitis., Ethanol, Glucose, Mutants

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Sreenath, Hassan K.; Jeffries, Thomas W.  1998.  2-deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis   Twentieth symposium on biotechnology for fuels and chemicals : proceedings of the twentieth symposium on biotechnology for fuels and chemicals held May 3-7, 1998, in Gatlinburg, Tennessee. Totowa, N.J. : Humana Press, 1999. Applied biochemistry and biotechnology, 0273-2280 ; vols. 77-79.:p. 211-222..