Title:	Detection and quantification of Leptographium wageneri, the cause of black-stain root disease, from bark beetles (Coleoptera: Scolytidae) in North California using regular and real-time PCR
Author(s):	Schweigkofler, Wolfgang; Otrosina, William J.; Smith, Sheri L.; Cluck, Daniel R.; Maeda, Kevin; Peay, Kabir G.; Garbelotto, Matteo
Date:	2005
Source:	Can. J. For. Res., Vol. 35: 1798-1808
Description:	Black-stain root disease is a threat to conifer forests in western North America. The disease is caused by the ophiostomatoid fungus Leptographium wageneri (W.B. Kendr.) M.J. Wingf., which is associated with a number of bark beetle (Coleoptera: Scolytidae) and weevil species (Coleoptera: Curculionidae). We developed a polymerase chain reaction test to identify and quantify fungal DNA directly from insects. Leptographium wageneri DNA was detected on 142 of 384 bark beetle samples (37%) collected in Lassen National Forest, in northeastern California, during the years 2001 and 2002. Hylastes macer (LeConte) was the bark beetle species from which Leptographium DNA was amplified most regularly (2001: 63.4%, 2002: 75.0% of samples). Lower insect-fungus association rates were found for Hylurgops porosus (LeConte), Hylurgops subcostulatus (Mannerheim), Hylastes gracilis (LeConte), Hylastes longicollis (Swaine),Dendroctonus valens (LeConte), and lps pini (Say). The spore load per beetle ranged from 0 to over 1 x l05 spores, with only a few beetles carrying more than 1 x 103 spores. The technique permits the processing of a large number of samples synchronously, as required for epidemiological studies, to study infection rates in bark beetle populations and to identify potential insect vectors.
	View and Print this Publication (1.19 MB)
Pristine Version:	An uncaptured or "pristine" version of this publication is available. It has not been subjected to OCR (Optical Character Recognition) and therefore does not have any errors in the text. However it is a larger file size and some people may experience long download times. The "pristine" version of this publication is available here: View and Print the PRISTINE copy of this Publication (1.44 MB)
Publication Notes:	We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information. This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain. Our on-line publications are scanned and captured using Adobe Acrobat. During the capture process some typographical errors may occur. Please contact the SRS Webmaster, srswebmaster@fs.fed.us if you notice any errors which make this publication unuseable.
	Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility