Antiserum Preparation For Immunodiffusion In Southern Pine Beetle Predation Studies

M.C. Miller; W. Adrian Chappell; William C. Gamble; J. Robert Bridges

Antiserum Preparation For Immunodiffusion In Southern Pine Beetle Predation Studies

Informally Refereed

Authors:

M.C. Miller, W. Adrian Chappell, William C. Gamble, J. Robert Bridges

Year:

1978

Type:

Research Note

Station:

Southern Research Station

Source:

Res. Note SO-233. New Orleans, LA: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station. 5 p.

Download PDF (111 KB)

Abstract

An anti-adult southern pine beetle serum was produced by subcutaneous injection of rabbits with southern pine beetle (SPB) adult antigen. Initial tests demonstrated the ability of the anti-adult SPB serum to detect adult SPB antigen in the body of the adult predator, Thanasimus dubius (F.). Cross reactivity was found between the anti-adult serum and extracts of immature stages of Dendroctonus frontalis Zimmerman, adult D. terebrans (Olivier), lps grandicollis (Eichhoff), and I. calligraphus (Germar). Adult southern pine beetle protein can be detected in a whole predator extract at least 28 hours after feeding.

Keywords

Dendroctonus frontalis, southern pine beetle, Thanasimus dubius, Agar gel double diffusion, precipitin test, predation

Citation

Miller, M.C.; Chappell, W. Adrian; Gamble, William C.; Bridges, J. Robert. 1978. Antiserum Preparation For Immunodiffusion In Southern Pine Beetle Predation Studies. Res. Note SO-233. New Orleans, LA: U.S. Department of Agriculture, Forest Service, Southern Forest Experiment Station. 5 p.